Infection of In Vivo and In Vitro Pines with the Pinewood Nematode Bursaphelenchus xylophilus and Isolation of Induced VolatilesJorge M. S. Faria 1,2, A. Cristina Figueiredo 3,4, Dora M. Teixeira 5, Maria L. Inácio 1,2
1INIAV, I.P., National Institute for Agrarian and Veterinary Research, I.P., 2GREEN-IT Bioresources for Sustainability, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa (ITQB NOVA), 3Centre for Environmental and Marine Studies (CESAM Lisboa), Biotecnologia Vegetal (BV), DBV, Faculdade de Ciências da Universidade de Lisboa, 4Centre for Ecology, Evolution and Environmental Changes (CE3C), Biotecnologia Vegetal (BV), DBV, Faculdade de Ciências da Universidade de Lisboa, 5Hercules Laboratory & Chemistry and Biochemistry Department of Science and Technology School, University of Évora
The protocol describes the in vivo and in vitro pinewood nematode infection of Pinus pinaster and their volatilome analysis through Gas Chromatography (GC) and GC coupled to Mass Spectrometry (GC-MS).
