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University of California at Berkeley

4 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells

Meghan Zuck^1,2, Caroline Feng², Kevin Hybiske¹

¹Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, ²Program in Infectious Diseases, School of Public Health, University of California at Berkeley

A live cell fluorescent protein based method for illuminating cellular vacuoles (inclusions) containing Chlamydia is described. This strategy enables rapid, automated determination of Chlamydia infectivity in samples and can be used to quantitatively investigate inclusion growth dynamics.

Engineering

Fabrication of Gate-tunable Graphene Devices for Scanning Tunneling Microscopy Studies with Coulomb Impurities

Han Sae Jung^1,2, Hsin-Zon Tsai¹, Dillon Wong¹, Chad Germany¹, Salman Kahn¹, Youngkyou Kim^1,3, Andrew S. Aikawa¹, Dhruv K. Desai¹, Griffin F. Rodgers¹, Aaron J. Bradley¹, Jairo Velasco Jr.¹, Kenji Watanabe⁴, Takashi Taniguchi⁴, Feng Wang^1,5,6, Alex Zettl^1,5,6, Michael F. Crommie^1,5,6

¹Department of Physics, University of California at Berkeley, ²Department of Chemistry, University of California at Berkeley, ³Department of Chemical and Biomolecular Engineering, University of California at Berkeley, ⁴National Institute for Materials Science (Japan), ⁵Materials Sciences Division, Lawrence Berkeley National Laboratory, ⁶Kavli Energy NanoSciences Institute, University of California at Berkeley and Lawrence Berkeley National Laboratory

This paper details the fabrication process of a gate-tunable graphene device, decorated with Coulomb impurities for scanning tunneling microscopy studies. Mapping the spatially dependent electronic structure of graphene in the presence of charged impurities unveils the unique behavior of its relativistic charge carriers in response to a local Coulomb potential.

Medicine

Real-Time Dynamic Navigation System for the Precise Quad-Zygomatic Implant Placement in a Patient with a Severely Atrophic Maxilla

Yihan Shen^*1, Qinggang Dai^*1, Baoxin Tao^*1, Wei Huang², Feng Wang², Kengliang Lan¹, Yuanyuan Sun¹, Xiaowan Ling¹, Lijun Yan¹, Yueping Wang¹, Yiqun Wu¹

¹The 2nd Dental Center, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, ²Department of Dental Implantology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology

Here, we present a protocol to achieve precise quad-zygomatic implant placement in patients with severely atrophic maxilla using a real-time dynamic navigation system.

Biology

Measuring In Vivo Adipose Tissue Kinetics in Humans Using the Deuterium (²H)-Labeling Approach

Dereck Kibodeaux¹, Jonathan Savoie¹, Eric Ravussin¹, Mark Fitch², Marc K. Hellerstein², Ursula White¹

¹Pennington Biomedical Research Center, Louisiana State University System, ²University of California at Berkeley

Presented here is a protocol to measure in vivo adipose tissue kinetics in humans using the deuterium (²H)-labeling method.