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Otto-von-Guericke University

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Direct Observation of Phagocytosis and NET-formation by Neutrophils in Infected Lungs using 2-photon Microscopy

Mike Hasenberg¹, Anja Köhler¹, Susanne Bonifatius¹, Andreas Jeron², Matthias Gunzer¹

¹Institute for Molecular and Clinical Immunology, Otto-von-Guericke University Magdeburg, ²Department of Immunoregulation, Helmholtz Center for Infection Research

We show, how to use 2-photon microscopy for the observation of the dynamics of neutrophil granulocytes in infected lungs while they phagocytose pathogens or produce neutrophil extracellular traps (NETs).

Immunology and Infection

Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies

Marcus Gereke^1,2, Andrea Autengruber^1,2, Lothar Gröbe³, Andreas Jeron², Dunja Bruder^1,2, Sabine Stegemann-Koniszewski¹

¹Research Group Immune Regulation, Helmholtz Centre for Infection Research, ²Research Group Infection Immunology, Institute of Medical Microbiology, Otto-von-Guericke University , ³Department of Experimental Immunology, Helmholtz Centre for Infection Research

We describe the rapid isolation of primary murine type II alveolar epithelial cells (AECII) by flow cytometric negative selection. These AECII show high viability and purity and are suitable for a wide range of functional and molecular studies regarding their role in respiratory conditions such as autoimmune or infectious diseases.

Cancer Research

Chemical Conjugation of a Purified DEC-205-Directed Antibody with Full-Length Protein for Targeting Mouse Dendritic Cells In Vitro and In Vivo

Julia Volckmar^1,2, Laura Knop^1,2,3, Tatjana Hirsch¹, Sarah Frentzel^1,2, Christian Erck⁴, Marco van Ham⁴, Sabine Stegemann-Koniszewski^*1,2,5, Dunja Bruder^*1,2

¹Immune Regulation Group, Helmholtz Centre for Infection Research, ²Infection Immunology Group, Institute of Medical Microbiology, Infection Prevention and Control, Health Campus Immunology, Infectiology and Inflammation, Otto-von-Guericke University Magdeburg, ³Institute for Molecular and Clinical Immunology, Health Campus Immunology, Infectiology and Inflammation, Otto-von-Guericke University Magdeburg, ⁴Cellular Proteome Research, Helmholtz Centre for Infection Research, ⁵Experimental Pneumology, University Hospital of Pneumology, Health Campus Immunology, Infectiology and Inflammation, Otto-von-Guericke University Magdeburg

We describe a protocol for the chemical conjugation of the model antigen ovalbumin to an endocytosis receptor-specific antibody for in vivo dendritic cell targeting. The protocol includes purification of the antibody, chemical conjugation of the antigen, as well as purification of the conjugate and the verification of efficient conjugation.