Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based MetabolomicsAnamika Singh 1,2, Mohd Adnan Siddiqui 1, Afzal Azim 3, Neeraj Sinha 1,2
1Centre of Biomedical Research, SGPGIMS Campus, 2Academy of Scientific and Innovative Research (AcSIR), 3Department of Critical Care Medicine, Sanjay Gandhi Postgraduate Institute of Medical Sciences
Nuclear magnetic resonance (NMR) spectroscopy is used to identify dysregulation in the metabolites in patients with various diseases. This technique allows the quantification of the deranged metabolites, unraveling the pathophysiological insights. Here, we describe the step-by-step procedure of the NMR-based approach for the metabolic characterization of the patients.
