IMPM

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

GC-MS Analysis of Deuterium Enrichment of Retinol in Serum and Estimation of Vitamin A Total Body Stores

¹Centre for Food and Nutrition Research, IMPM

This method involves extracting retinol from serum, separating it using HPLC, and determining labeled and non-labeled retinol isotopes using GC-MS. The ratio of labeled to non-labeled retinol is used to estimate total body stores of vitamin A.