Rapid in vivo Drug Response Prediction Using Leukemia Cell Grafts in Zebrafish EmbryosSophia Sartorius 1, Luca Bramè 1,2, Jutta Proba 1, Anton Gauert 1, Nadine Olk 1, Juan Lazaro 1, Angelika Eggert 1, Cornelia Eckert 1, Anja H. Hagemann 1,2
1Charité-Universitätsmedizin Berlin, Klinik für Pädiatrie m.S. Onkologie/Hämatologie, 2German Cancer Consortium (DKTK), partner site Berlin, and German Cancer Research Center (DKFZ)
This protocol provides step-by-step instructions for generating and troubleshooting human acute lymphoblastic leukemia (ALL) xenografts from cell lines and fresh patient material in transiently immunosuppressed zebrafish embryos, along with guidelines for drug response assessment using flow cytometry. The experimental pipeline can also be adapted for solid tumors.
