CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection DeliveryAnna E. Christenson *1, Nikita S. Divekar *1, Mitzi G. Hernández Zamora 1, Carolyn D. Nguyen 1, James K. Nuñez 1,2
1Department of Molecular and Cell Biology, University of California, Berkeley, 2Chan Zuckerberg Biohub - San Francisco
The protocol describes methods for Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-based epigenome editing in human cell lines using plasmid DNA transfection and mRNA nucleofection.
