Collection, Expansion, and Differentiation of Primary Human Nasal Epithelial Cell Models for Quantification of Cilia Beat FrequencyKatelin M. Allan 1,2, Sharon L. Wong 1,2, Laura K. Fawcett 1,2,3, Alexander Capraro 1,2, Adam Jaffe 1,2,3, Cristan Herbert 4, Elvis Pandzic 5, Shafagh A. Waters 1,2,3
1School of Women's and Children's Health, Faculty of Medicine and Health, University of New South Wales, 2Molecular and Integrative Cystic Fibrosis Research Centre (miCF RC), Faculty of Medicine and Health, University of New South Wales, 3Department of Respiratory Medicine, Sydney Children's Hospital, 4Department of Pathology, School of Medical Sciences, University of New South Wales Australia, 5Katharina Gaus Light Microscopy Facility, Mark Wainwright Analytical Centre, University of New South Wales
This protocol describes nasal epithelial cell collection, expansion, and differentiation to organotypic airway epithelial cell models and quantification of cilia beat frequency via live-cell imaging and custom-built scripts.
