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Tulane National Primate Research Center

4 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Recognition of Epidermal Transglutaminase by IgA and Tissue Transglutaminase 2 Antibodies in a Rare Case of Rhesus Dermatitis

Karol Sestak¹, Kaushiki Mazumdar¹, Cecily C. Midkiff², Jason Dufour³, Juan T. Borda², Xavier Alvarez²

¹Division of Microbiology, Tulane National Primate Research Center, ²Division of Comparative Pathology, Tulane National Primate Research Center, ³Division of Veterinary Medicine, Tulane National Primate Research Center

Dermatitis herpetiformis (DH) is a chronic inflammatory condition characterized by an autoimmune reaction between IgA and epidermal transglutaminase (eTG). DH develops in a very small portion of gluten-sensitive and/or celiac patients. The results of this study indicate that DH can also develop in a rhesus monkey host with symptoms of idiopatic dermatitis.

Bioengineering

Nonhuman Primate Lung Decellularization and Recellularization Using a Specialized Large-organ Bioreactor

Ryan W. Bonvillain^1,2, Michelle E. Scarritt¹, Nicholas C. Pashos¹, Jacques P. Mayeux¹, Christopher L. Meshberger¹, Aline M. Betancourt^1,3, Deborah E. Sullivan^1,3, Bruce A. Bunnell^1,2,4

¹Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, ²Division of Regenerative Medicine, Tulane National Primate Research Center, ³Department of Microbiology and Immunology, Tulane University School of Medicine, ⁴Department of Pharmacology, Tulane University School of Medicine

Whole-organ decellularization produces natural biological scaffolds that may be used for regenerative medicine. The description of a nonhuman primate model of lung regeneration in which whole lungs are decellularized and then seeded with adult stem cells and endothelial cells in a bioreactor that facilitates vascular circulation and liquid media ventilation is presented.

Immunology and Infection

A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas

Teresa A. Hudock¹, Deepak Kaushal^1,2

¹Bacteriology and Parasitology, Tulane National Primate Research Center, ²Microbiology and Immunology, Tulane National Primate Research Center

Microdissection has been extensively employed for the examination of DNA, RNA, and protein within tissue. Laser capture microscopy (LCM) is the most commonly used method, but a new milling technique, mesodissection, is recently available. We demonstrate RNA extraction from mesodissected formalin fixed paraffin embedded tissue slides of Mycobacterium tuberculosis granulomas.

Bioengineering

Creation of a Knee Joint-on-a-Chip for Modeling Joint Diseases and Testing Drugs

Meagan J. Makarcyzk^1,2, Zhong Alan Li^1,3, Ilhan Yu¹, Haruyo Yagi¹, Xiurui Zhang¹, Lauren Yocum¹, Eileen Li¹, Madalyn R. Fritch¹, Qi Gao⁴, Bruce A. Bunnell⁵, Stuart B. Goodman^4,6, Rocky S. Tuan^1,8, Peter G. Alexander^1,7, Hang Lin^1,2,7

¹Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, ²Department of Bioengineering, University of Pittsburgh Swanson School of Engineering, ³Department of Neurobiology, University of Pittsburgh School of Medicine, ⁴Department of Orthopaedic Surgery, Stanford University, ⁵Department of Microbiology, Immunology, and Genetics, University of North Texas Health Science Center, ⁶Department of Bioengineering, Stanford University, ⁷McGowan Institute for Regenerative Medicine, University of Pittsburgh School of Medicine, ⁸The Chinese University of Hong Kong

We provide detailed methods for generating four types of tissues from human mesenchymal stem cells, which are used to recapitulate the cartilage, bone, fat pad, and synovium in the human knee joint. These four tissues are integrated into a customized bioreactor and connected through microfluidics, thus generating a knee joint-on-a-chip.