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NASA Ames Research Center

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research

Wan W. Amani Wan Salim¹, Michael A. Zeitchek¹, Andrew C. Hermann¹, Antonio J. Ricco², Ming Tan², Florian Selch², Erich Fleming², Brad M. Bebout², Mamoun M. Bader³, Aeraj ul Haque⁴, D. Marshall Porterfield⁵

¹Department of Agricultural and Biological Engineering, Birck-Bindley Physiological Sensing Facility, Purdue University, ²NASA Ames Research Center, ³Department of Chemistry, Pennsylvania State University Hazleton, ⁴Cooley LLP, ⁵NASA Life and Physical Sciences, Human Exploration and Operations Mission Directorate, NASA Headquarters

All-solid-state ion-selective electrodes (ASSISEs) constructed from a conductive polymer (CP) transducer provide several months of functional lifetime in liquid media. Here, we describe the fabrication and calibration process of ASSISEs in a lab-on-a-chip format. The ASSISE is demonstrated to have maintained a near-Nernstian slope profile after prolonged storage in complex biological media.

Genetics

Exploring the Effects of Spaceflight on Mouse Physiology using the Open Access NASA GeneLab Platform

Afshin Beheshti¹, Yasaman Shirazi-Fard², Sungshin Choi¹, Daniel Berrios³, Samrawit G. Gebre¹, Jonathan M. Galazka², Sylvain V. Costes²

¹WYLE Labs, Space Biosciences Division, NASA Ames Research Center, ²Space Biosciences Division, NASA Ames Research Center, ³USRA, NASA Ames Research Center

The NASA GeneLab platform provides unfettered access to precious omics data from biological spaceflight experiments. We describe how a typical mouse experiment is conducted in space and how data from such experiments can be accessed and analyzed.

JoVE Core

Collecting Sleep, Circadian, Fatigue, and Performance Data in Complex Operational Environments

Lucia Arsintescu¹, Kenji H. Kato², Cassie J. Hilditch¹, Kevin B. Gregory¹, Erin Flynn-Evans³

¹San Jose State University Research Foundation, ²ASRS Research and Technology Solutions, ³Fatigue Countermeasures Laboratory, NASA Ames Research Center

Sleep loss and circadian misalignment contribute to numerous operational accidents and incidents. The effectiveness of countermeasures and work scheduling designs aimed at mitigating fatigue can be challenging to evaluate in operational environments. This manuscript summarizes an approach for collecting sleep, circadian, fatigue, and performance data in complex operational environments.