Twincore, Centre for Experimental and Clinical Infection Research
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors
Anne Frentzen *1, Kathrin Hueging *1, Julia Bitzegeio 2, Thomas Pietschmann 1, Eike Steinmann 1
1Division of Experimental Virology, Twincore, Centre for Experimental and Clinical Infection Research, 2Aaron Diamond AIDS Research Center, Laboratory of Retrovirology, The Rockefeller University, NY
We describe two methods for conditional trans-complementation of hepatitis C virus (HCV) assembly and the completion of the full viral life cycle, which rely on heterokaryon formation. These techniques are suitable to screen for cell lines that express dominant restriction factors, which preclude production of infectious HCV progeny.
Immunology and Infection
A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks
Toni Luise Meister *1, Mara Klöhn *1, Eike Steinmann 1, Daniel Todt 1
1Molecular and Medical Virology, Ruhr University Bochum
Described here is an effective method on how to produce high viral titer stocks of hepatitis E virus (HEV) to efficiently infect hepatoma cells. With the presented method, both non-enveloped, as well as enveloped viral particles can be harvested and used for inoculating various cell lines.
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