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Baltimore Va Medical Center

3 ARTICLES PUBLISHED IN JoVE

Biology

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Karthika Natarajan^1,2, Yi Xie^1,3, Takeo Nakanishi⁴, Rebecca S. Moreci^5,6, Pancharatnam Jeyasuria⁷, Arif Hussain^1,3,8,9, Douglas D. Ross^{1,3,8,9,10,11}

¹Greenebaum Cancer Center, University of Maryland School of Medicine, ²Pharmaceutical Sciences, University of Maryland School of Pharmacy, ³Baltimore VA Medical Center, ⁴Membrane Transport and Biopharmaceutics, School of Pharmaceutical Sciences, Kanazawa University, ⁵Obstetrics, Gynecology and Reproductive Science, University of Pittsburgh, ⁶Magee Women's Research Institute, ⁷Obstetrics, Gynecology, Perinatal Research Branch (NICHD), Wayne State University School of Medicine, ⁸Medicine, University of Maryland School of Medicine, ⁹Pathology, University of Maryland School of Medicine, ¹⁰Pharmacology, University of Maryland School of Medicine, ¹¹Experimental Therapeutics, University of Maryland School of Medicine

With the murine ABC transporter Bcrp1 (Abcg2) as an example, in-silico protocols are presented to detect alternative promoter usage in genes expressed in mouse tissues, and to evaluate the functionality of the alternative promoters identified using reporter assays.

Medicine

Proton Therapy Delivery and Its Clinical Application in Select Solid Tumor Malignancies

Adeel Kaiser¹, John G. Eley¹, Nasarachi E. Onyeuku¹, Stephanie R. Rice¹, Carleen C. Wright², Nathan E. McGovern², Megan Sank², Mingyao Zhu¹, Zeljko Vujaskovic¹, Charles B. Simone 2nd¹, Arif Hussain³

¹Department of Radiation Oncology, University of Maryland School of Medicine, ²Department of Radiation Oncology, University of Maryland Medical Center, ³Department of Medicine, University of Maryland School of Medicine, University of Maryland Greenebaum Comprehensive Cancer Center

The fundamentals of radiation planning and delivery for proton therapy using prostate cancer as a model are presented. The application of these principles to other selected disease sites highlights how proton radiotherapy may enhance clinical outcomes for cancer patients.

Behavior

A Chronic Sleep Fragmentation Model using Vibrating Orbital Rotor to Induce Cognitive Deficit and Anxiety-Like Behavior in Young Wild-Type Mice

Yi Xie¹, Sai-Yue Deng¹, Si-Miao Chen¹, Xue-Jiao Chen¹, Wen-Wen Lai¹, Li-Fang Huang¹, Li Ba¹, Wei Wang^1,2, Feng-Fei Ding^1,3

¹Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, China, ²Key Laboratory of Neurological Diseases of Chinese Ministry of Education, The School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000,China, ³Department of Pharmacology, School of Basic Medical Science; State Key Laboratory of Medical Neurobiology, Institutes of Brain Science and Collaborative Innovation Center for Brain Science, Fudan University, Shanghai 200032, China

Presented here is a protocol for chronic sleep fragmentation (CSF) model achieved by an electrically controlled orbital rotor, which could induce confirmed cognitive deficit and anxiety-like behavior in young wild-type mice. This model can be applied to explore the pathogenesis of chronic sleep disturbance and related disorders.