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College of Medicine, The Ohio State University

2 ARTICLES PUBLISHED IN JoVE

Biology

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps

Bhavani Gopalakrishnan¹, Kevin M. Nash¹, Murugesan Velayutham¹, Frederick A. Villamena^1,2

¹The Davis Heart and Lung Research Institute, The Ohio State University, ²Department of Pharmacology, College of Medicine, The Ohio State University

Electron paramagnetic resonance (EPR) spectroscopy was employed to detect nitric oxide from bovine aortic endothelial cells and superoxide radical anion from human neutrophils using iron (II)-N-methyl-D-glucamine dithiocarbamate, Fe(MGD)₂ and 5,5-dimethyl-1-pyroroline-N-oxide, DMPO, respectively.

Neuroscience

In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes

Cassandra N. Dennys¹, Julieth A. Sierra-Delgado¹, Shrestha Sinha Ray¹, Annalisa M. Hartlaub¹, Florence S. Roussel¹, Yacidzohara Rodriguez¹, Kathrin Meyer^1,2

¹Center for Gene Therapy, The Research Institute at Nationwide Children’s Hospital, ²College of Medicine, The Ohio State University

We describe a protocol to reprogram human skin-derived fibroblasts into induced Neuronal Progenitor Cells (iNPCs), and their subsequent differentiation into induced Astrocytes (iAs). This method leads to fast and reproducible generation of iNPCs and iAs in large quantities.