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Eindhoven University of Technology, The Netherlands

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation

Daisy W. J. van der Schaft¹, Ariane C. C. van Spreeuwel¹, Kristel J. M. Boonen¹, Marloes L. P. Langelaan¹, Carlijn V. C. Bouten¹, Frank P. T. Baaijens¹

¹Department of Biomedical Engineering, Soft Tissue Biomechanics and Engineering, Eindhoven University of Technology, The Netherlands

Engineered muscle tissue has great potential in regenerative medicine, as disease model and also as an alternative source for meat. Here we describe the engineering of a muscle construct, in this case from mouse myoblast progenitor cells, and the stimulation by electrical pulses.

Bioengineering

Engineering Fibrin-based Tissue Constructs from Myofibroblasts and Application of Constraints and Strain to Induce Cell and Collagen Reorganization

Nicky de Jonge¹, Frank P. T. Baaijens¹, Carlijn V. C. Bouten¹

¹Department of Biomedical Engineering, Eindhoven University of Technology

This model system starts from a myofibroblast-populated fibrin gel that can be used to study endogenous collagen (re)organization real-time in a nondestructive manner. The model system is very tunable, as it can be used with different cell sources, medium additives, and can be adapted easily to specific needs.

Bioengineering

Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates

Cas van der Putten^1,2, Mirko D’Urso^1,2, Maaike Bril^1,2, Thomas E. Woud^1,2, Carlijn V. C. Bouten^1,2, Nicholas A. Kurniawan^1,2

¹Department of Biomedical Engineering, Eindhoven University of Technology, ²Institute for Complex Molecular Systems, Eindhoven University of Technology

Traditionally, cell culture is performed on planar substrates that poorly mimic the natural environment of cells in vivo. Here we describe a method to produce cell culture substrates with physiologically relevant curved geometries and micropatterned extracellular proteins, allowing systematic investigations into cellular sensing of these extracellular cues.