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University of Halle

2 ARTICLES PUBLISHED IN JoVE

Medicine

Coculture System with an Organotypic Brain Slice and 3D Spheroid of Carcinoma Cells

Han-Ning Chuang¹, Raphaela Lohaus¹, Uwe-Karsten Hanisch², Claudia Binder¹, Faramarz Dehghani^*3, Tobias Pukrop^*1

¹Department of Hematology and Oncology, University of Göttingen, ²Institute of Neuropathology, University of Göttingen, ³Institute of Anatomy and Cellbiology, University of Halle

The organotypic brain slice coculture with carcinoma cells enables visualizing morphological changes by fluorescence as well as bright field (video) microscopy during the process of carcinoma cell invasion of brain tissue. This model system also allows for cell exchange and replenishment approaches and offers a wide variety of manipulations and analyses.

Neuroscience

Organotypic Hippocampal Slice Cultures As a Model to Study Neuroprotection and Invasiveness of Tumor Cells

Urszula Grabiec^*1, Tim Hohmann^*1, Niels Hammer², Faramarz Dehghani¹

¹Department of Anatomy and Cell Biology, Martin Luther University Halle-Wittenberg, ²Department of Anatomy, University of Otago

Organotypic hippocampal slice cultures (OHSC) represent an in vitro model that simulates the in vivo situation very well. Here we describe a vibratome-based improved slicing protocol to obtain high quality slices for use in assessing the neuroprotective potential of novel substances or the biological behavior of tumor cells.