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Harvard School of Engineering and Applied Sciences

2 ARTICLES PUBLISHED IN JoVE

Biology

Intraductal Injection for Localized Drug Delivery to the Mouse Mammary Gland

Silva Krause¹, Amy Brock², Donald E. Ingber^1,2,3

¹Vascular Biology Program, Department of Surgery, Boston Children's Hospital and Harvard Medical School, ²Wyss Institute for Biologically Inspired Engineering, Harvard University, ³Harvard School of Engineering and Applied Sciences

A protocol for the non-invasive intraductal delivery of aqueous reagents to the mouse mammary gland is described. The method takes advantage of localized injection into the nipples of mammary glands targeting mammary ducts specifically. This technique is adaptable for a variety of compounds including siRNA, chemotherapeutic agents and small molecules.

Bioengineering

Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device

Hyun Jung Kim¹, Jaewon Lee¹, Jin-Ha Choi¹, Anthony Bahinski², Donald E. Ingber^2,3,4

¹Department of Biomedical Engineering, The University of Texas at Austin, ²Wyss Institute for Biologically Inspired Engineering at Harvard University, ³Vascular Biology Program, Boston Children's Hospital, Harvard Medical School, ⁴John A. Paulson School of Engineering and Applied Sciences, Harvard University

We describe an in vitro protocol to co-culture gut microbiome and intestinal villi for an extended period using a human gut-on-a-chip microphysiological system.