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Korea Research Institute of Bioscience and Biotechnology (KRIBB)

2 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat

Haeyoung Jeong^*1, Sang J. Lee^*2, Pil Kim³

¹Super-Bacteria Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), ²Microbiomics and Immunity Research Center, Korea Research Institute of Bioscience and Bioengineering (KRIBB), ³Department of Biotechnology, The Catholic University of Korea

Here, we present a protocol to obtain adaptive laboratory evolution of microorganisms under conditions using chemostat culture. Also, genomic analysis of the evolved strain is discussed.

Cancer Research

Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells

Jina Lee^*1,2, Joo-Eun Lee^*3, Seil Kim^1,4,5, Dukjin Kang¹, Hee Min Yoo¹

¹Microbiological Analysis Team, Group for Biometrology, Korea Research Institute of Standards and Science (KRISS), ²College of Pharmacy, Chungnam National University (CNU), ³Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), ⁴Convergent Research Center for Emerging Virus Infection, Korea Research Institute of Chemical Technology (KRICT), ⁵Department of Bio-Analysis Science, University of Science & Technology (UST)

Here methods are presented to understand anti-cancer effects of Lactobacillus cell-free supernatant (LCFS). Colorectal cancer cell lines show cell deaths when treated with LCFS in 3D cultures. The process of generating spheroids can be optimized depending on the scaffold and the analysis methods presented are useful for evaluating the involved signaling pathways.