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Pitzer College

3 ARTICLES PUBLISHED IN JoVE

Engineering

Drawing and Hydrophobicity-patterning Long Polydimethylsiloxane Silicone Filaments

Katherine Snell¹, Isabelle Lopez², Brandon Louie¹, Roxanna Kiessling¹, Babak Sanii^1,2,3

¹Keck Science Department, Claremont McKenna College, ²Keck Science Department, Scripps College, ³Keck Science Department, Pitzer College

Here, we present a protocol to produce long filaments of polydimethylsiloxane (PDMS) silicone by gravity-drawing through a furnace. Filaments are on the order of hundreds of micrometers in diameter and tens of centimeters in length and are hydrophobically patternable via an Arduino-controlled corona discharge system.

Biology

Analysis of Motility Patterns of Stentor During and After Oral Apparatus Regeneration Using Cell Tracking

Janet Y. Sheung¹, Megan Otsuka², Gabriella Seifert³, Athena Lin⁴, Wallace F. Marshall⁴

¹W. M. Keck Science Department, Scripps, Pitzer, and Claremont McKenna of The Claremont Colleges, ²W. M. Keck Science Department, Pitzer College, ³W. M. Keck Science Department, Scripps College, ⁴Department of Biochemistry and Biophysics, School of Medicine, University of California at San Francisco

We present a protocol for the characterization of motility and behavior of a population of hundred micron- to millimeter-sized cells using brightfield microscopy and cell tracking. This assay reveals that Stentor coeruleus transitions through four behaviorally distinct phases when regenerating a lost oral apparatus.

Design and Building of a Customizable, Single-Objective, Light-Sheet Fluorescence Microscope for the Visualization of Cytoskeleton Networks

Nathan Felcher¹, Daisy Achiriloaie¹, Brian Lee², Ryan McGorty³, Janet Sheung^1,2,4

¹W.M. Keck Science Department, Scripps College, ²W.M. Keck Science Department, Claremont McKenna College, ³Department of Physics and Biophysics, University of San Diego, ⁴W.M. Keck Science Department, Pitzer College

This protocol describes in detail how to build a single-objective, light-sheet fluorescence microscope and its usage for visualizing cytoskeleton networks.