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The First Affiliated Hospital of Nanchang University

3 ARTICLES PUBLISHED IN JoVE

Medicine

Modeling Myotonic Dystrophy 1 in C2C12 Myoblast Cells

Rui Liang^*1, Wei Dong^*1,3, Xiaopeng Shen¹, Xiaoping Peng^1,3, Angie G. Aceves^1,2, Yu Liu¹

¹Department of Biology and Biochemistry, University of Houston, ²Department of Economics, University of Houston, ³Department of Cardiology, The First Affiliated Hospital of Nanchang University

In this protocol, we present the procedures in establishing myotonic dystrophy 1 myoblast models, including optimized C2C12 cell maintenance, gene transfection/transduction, and myocyte differentiation.

Biochemistry

Carrier-assisted One-pot Sample Preparation for Targeted Proteomics Analysis of Small Numbers of Human Cells

Kendall Martin¹, Tong Zhang¹, Pengfei Zhang², William B. Chrisler¹, Fillmore L. Thomas³, Fen Liu⁴, Tao Liu¹, Wei-Jun Qian¹, Richard D. Smith¹, Tujin Shi¹

¹Biological Sciences Division, Pacific Northwest National Laboratory, ²NHC Key Laboratory of Cancer Proteomics, Department of Oncology, Xiangya Hospital, Central South University, ³Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, ⁴Department of Critical Care Medicine, The First Affiliated Hospital of Nanchang University

A protein carrier-assisted one-pot sample preparation coupled with liquid chromatography (LC) - selected reaction monitoring (SRM) termed cLC-SRM is a convenient method for multiplexed targeted proteomics analysis of small numbers of cells, including single cells. It capitalizes on using excessive exogenous protein as a carrier and high-specificity LC-SRM for targeted quantification.

Biochemistry

Preparation of Mechanically Stable Self-Assembled Peptides Hydrogels

Yue Zhao¹, Mai Hu², Yiwen Gong², Yihao Zhao², Zhiwei Shen²

¹The First Affiliated Hospital of Nanchang University, ²The National Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Nanchang University

This protocol presents three fast and simple preparation methods that use environmental conditions to trigger the self-assembly of peptides into hydrogels. Additionally, the characterization of peptide hydrogels is described, demonstrating that mechanically stable peptide hydrogels can be formed under these straightforward conditions.