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Mayo Clinic College of Medicine and Science

1 ARTICLES PUBLISHED IN JoVE

Cancer Research

Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells

Rosalie M. Sterner^1,2, Michelle J. Cox³, Reona Sakemura³, Saad S. Kenderian^2,3

¹Mayo Clinic Medical Scientist Training Program, Mayo Clinic College of Medicine and Science, ²Department of Immunology, Mayo Clinic, ³Division of Hematology, Mayo Clinic

Here, we present a protocol to genetically edit CAR-T cells via a CRISPR/Cas9 system.

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