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University of Texas McGovern Medical School

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Technique to Target Microinjection to the Developing Xenopus Kidney

Bridget D. DeLay¹, Vanja Krneta-Stankic^1,2, Rachel K. Miller^1,2,3,4

¹Department of Pediatrics, Pediatric Research Center, University of Texas McGovern Medical School, ²Program in Genes & Development, University of Texas Graduate School of Biomedical Sciences, ³Program in Cell & Regulatory Biology, University of Texas Graduate School of Biomedical Sciences, ⁴Department of Genetics, University of Texas MD Anderson Cancer Center

Here, we present a protocol to use fate maps and lineage tracers to target injections into individual blastomeres that give rise to the kidney of Xenopus laevis embryos.

Medicine

Preclinical Model of Hind Limb Ischemia in Diabetic Rabbits

Andrew D. Sligar¹, Gretchen Howe², Julia Goldman³, Patricia Felli², Varsha Karanam¹, Richard W. Smalling^2,4, Aaron B. Baker^1,5,6,7

¹Department of Biomedical Engineering, University of Texas at Austin, ²Division of Cardiology, University of Texas McGovern Medical School, ³Center for Laboratory Animal Medicine and Care, UT Health Science Center at Houston, ⁴Memorial Herman Heart and Vascular Center, Texas Medical Center, ⁵Institute for Cellular and Molecular Biology, University of Texas at Austin, ⁶The Institute for Computational Engineering and Sciences, University of Texas at Austin, ⁷Institute for Biomaterials, Drug Delivery and Regenerative Medicine, University of Texas at Austin

We describe a surgical procedure used to induce peripheral ischemia in rabbits with hyperlipidemia and diabetes. This surgery acts as a preclinical model for conditions experienced in peripheral artery disease in patients. Angiography is also described as a means to measure the extent of introduced ischemia and recovery of perfusion.