EMBL: European Molecular Biology Laboratory
2 ARTICLES PUBLISHED IN JoVE
Developmental Biology
Transcriptional Analysis by Nascent RNA FISH of In Vivo Trophoblast Giant Cells or In Vitro Short-term Cultures of Ectoplacental Cone Explants
Catherine Corbel 1, Edith Heard 1
1Unité de Génétique et Biologie du Développement, Institut Curie, PSL Research University, CNRS UMR 3215, INSERM U934
Trophoblast giant cells (TGCs) play a key role in the placenta to ensure a healthy pregnancy. We present a protocol for assessing the transcriptional status of genes in TGCs by nascent fluorescent in situ hybridization on cryostat sections of post-implantation embryos or short-term cultures of embryonic day 7 ectoplacental cones.
Biology
Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
Antonia Hauth 1, Rafael Galupa 1, Nicolas Servant 2, Laura Villacorta 1, Kai Hauschulz 3, Joke Gerarda van Bemmel 4, Agnese Loda 1, Edith Heard 1,5
1EMBL: European Molecular Biology Laboratory, 2Institut Curie, 3Agilent Technologies, 4Genmab BV, 5Collège de France
This protocol describes the Capture Hi-C method used to characterize the 3D organization of megabased-sized targeted genomic regions at high-resolution, including boundaries of topologically associating domains (TADs) and long-range chromatin interactions between regulatory and other DNA sequence elements.
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