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3 ARTICLES PUBLISHED IN JoVE
Biology
A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography
Johannes Groen 1, Andrea Sorrentino 1, Lucía Aballe 1, Robert Oliete 1, Ricardo Valcárcel 1, Chidinma Okolo 2, Ilias Kounatidis 2, Maria Harkiolaki 2, Ana Joaquina Pérez-Berná 1, Eva Pereiro 1
1MISTRAL beamline, Alba Light Source, Cerdanyola del Valles, Spain, 2Beamline B24, Diamond Light Source, Harwell Science and Innovation Campus, Didcot, UK
Here, a protocol describing the sample preparation and data collection steps required in cryo soft X-ray tomography (SXT) to image the ultrastructure of whole cryo-preserved cells at a resolution of 25 nm half pitch, is presented.
Biology
Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells
Nina Vyas *1, Nina Perry *1, Chidinma A. Okolo 1, Ilias Kounatidis 1, Thomas M. Fish 1, Kamal L. Nahas 1,2, Archana Jadhav 1, Mohamed A. Koronfel 1, Johannes Groen 3, Eva Pereiro 3, Ian M. Dobbie 4, Maria Harkiolaki 1
1Harwell Science and Innovation Campus, Beamline B24, Diamond Light Source, 2Division of Virology, Department of Pathology, University of Cambridge, 3ALBA Synchrotron, Beamline 09 - MISTRAL, 4Micron Advanced Imaging Consortium, Department of Biochemistry, University of Oxford
This protocol demonstrates how to image biological cryo-preserved samples using cryo-structured illumination microscopy. We demonstrate the methodology by imaging the cytoskeleton of U2OS cells.
iNEXT-Discovery and Instruct-ERIC: Integrating High-End Services for Translational Research in Structural Biology
Hans Wienk 1, Lucia Banci 2, Susan Daenke 3, Eva Pereiro 4, Harald Schwalbe 5, David Ian Stuart 6, Manfred S. Weiss 7, Anastassis Perrakis 1
1Division of Biochemistry and Oncode Institute, The Netherlands Cancer Institute, Amsterdam, 2CERM/CIRMMP, University of Florence, 3Instruct-ERIC, Oxford University, 4MISTRAL beamline, Alba Light Source, 5Center for Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, 6Division of Structural Biology, Oxford University, 7Macromolecular Crystallographyy, Helmholtz-Zentrum Berlin
iNEXT-Discovery and Instruct-ERIC: Integrating High-End Services for Translational Research in Structural Biology
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