Isolation and Genetic Manipulation of Adult Cardiac Myocytes for Confocal ImagingLars Kaestner 1, Anke Scholz 1, Karin Hammer 1, Anne Vecerdea 1, Sandra Ruppenthal 1, Peter Lipp 1
1Institute for Molecular Cell Biology, Universty of Saarland
Adult cardiac myocytes are primary cells that can be isolated from animal hearts and cultured for several days. Within this culture period adenoviral gene transfer can be used to express genetically encoded biosensors (GEBs) or fluorescent fusion proteins. Both approaches allow cellular investigations by means of confocal microscopy.
