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University of Bonn - Life & Brain Center and Hertie Foundation

2 ARTICLES PUBLISHED IN JoVE

Biology

Bernhard Münst¹, Christoph Patsch¹, Frank Edenhofer¹

¹Stem Cell Engineering Group, Institute of Reconstructive Neurobiology, University of Bonn - Life & Brain Center and Hertie Foundation

Protein transduction enables the direct delivery of biologically active proteins into cells. In contrast to conventional methods such as DNA transfection or viral transduction this non-invasive paradigm allows highly efficient cellular manipulation in a titratable manner circumventing cellular toxicity and the risk of oncogenic transformation by permanent genetic modification.

Neuroscience

Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells

Sandra Meyer^1,2, Philipp Wörsdörfer¹, Katharina Günther¹, Marc Thier^2,3, Frank Edenhofer^1,2

¹Institute of Anatomy and Cell Biology, University of Würzburg, ²Institute of Reconstructive Neurobiology, University of Bonn, ³German Cancer Research Center, Heidelberg

Generation of induced pluripotent stem cells provides fascinating prospects for the derivation of autologous transplants. However, progression through a pluripotent state and laborious re-differentiation still hinders clinical translation. Here we describe the derivation of adult human fibroblasts and their direct conversion into induced neural progenitor cells and the subsequent differentiation into neural lineages.