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Mount Sinai Hospital

4 ARTICLES PUBLISHED IN JoVE

Neuroscience

Assessment of Social Interaction Behaviors

Oksana Kaidanovich-Beilin¹, Tatiana Lipina¹, Igor Vukobradovic², John Roder^1,3,4,5, James R. Woodgett^1,3

¹Samuel Lunenfeld Research Institute, Mount Sinai Hospital, ²Toronto Centre for Phenogenomics, Mount Sinai Hospital, ³Department of Medical Biophysics, University of Toronto, ⁴Department of Psychology, University of Toronto, ⁵Department of Psychiatry, University of Toronto

Here we describe a detailed protocol for examination of sociability in mice by using Crawley's sociability and preference for social novelty test. We describe the advantages and possible applications for this procedure, including critical details important for correct interpretation of the results.

Developmental Biology

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

Enrica Bertin¹, Martina Piccoli¹, Chiara Franzin¹, Andras Nagy², Maria Mileikovsky², Paolo De Coppi³, Michela Pozzobon¹

¹Stem Cell and Regenerative Medicine Laboratory, Fondazione Istituto di Ricerca Pediatrica Citta della Speranza, ²Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, ³Stem Cells and Regenerative Medicine Section, Developmental Biology and Cancer Programme, UCL Institute of Child Health and Great Ormond Street Hospital

In this study, we generate induced pluripotent stem cells from mouse amniotic fluid cells, using a non-viral-based transposon system.

Cancer Research

An In Vivo Murine Sciatic Nerve Model of Perineural Invasion

Sylvie Deborde^*1, Yasong Yu^*1, Andrea Marcadis¹, Chun-Hao Chen¹, Ning Fan², Richard L. Bakst³, Richard J. Wong¹

¹Department of Surgery, Memorial Sloan Kettering Cancer Center, ²Molecular Cytology Core Facility, Memorial Sloan Kettering Cancer Center, ³Department of Radiation Oncology, Mount Sinai Hospital

We describe an in vivo murine model of perineural invasion by injecting syngeneic pancreatic cancer cells into the sciatic nerve. The model allows for quantification of the extent of nerve invasion, and supports investigation of the cellular and molecular mechanisms of perineural invasion.

Cancer Research

Magnetic Resonance-Guided High Intensity Focused Ultrasound Generated Hyperthermia: A Feasible Treatment Method in a Murine Rhabdomyosarcoma Model

Claire Wunker^1,2, Karolina Piorkowska³, Ben Keunen³, Yael Babichev², Suzanne M. Wong^3,4, Maximilian Regenold⁵, Michael Dunne⁵, Julia Nomikos^1,2, Maryam Siddiqui⁶, Samuel Pichardo⁶, Warren Foltz⁷, Adam C. Waspe^3,8, Justin T. Gerstle^3,9, James M. Drake^1,3,4,10, Rebecca A. Gladdy^1,2,10

¹Institute of Medical Science, University of Toronto, ²Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, ³The Wilfred and Joyce Posluns Centre for Image-Guided Innovation and Therapeutic Intervention, The Hospital for Sick Children, ⁴Institute of Biomedical Engineering, University of Toronto, ⁵Leslie Dan Faculty of Pharmacy, University of Toronto, ⁶Departments of Radiology and Clinical Neurosciences, University of Calgary, ⁷Department of Radiation Oncology, University of Toronto, ⁸Department of Medical Imaging, University of Toronto, ⁹Department of Pediatric Surgery, University of Toronto, ¹⁰Department of Surgery, University of Toronto

Presented here is a protocol to use controlled hyperthermia, generated by magnetic resonance-guided high intensity focused ultrasound, to trigger drug release from temperature-sensitive liposomes in a rhabdomyosarcoma mouse model.