National Institutes of Health, Bethesda
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Fluorescence Recovery After Photobleaching (FRAP) of Fluorescence Tagged Proteins in Dendritic Spines of Cultured Hippocampal Neurons
Chan-Ying Zheng 1, Ronald S. Petralia 1, Ya-Xian Wang 1, Bechara Kachar 1
1National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda
FRAP has been used to quantify the mobility of Green Fluorescence Protein (GFP)-tagged proteins in cultured cells. We examined the mobile/immobile fractions of the GFP by analyzing the fluorescence recovery percentage after photobleaching. In this study, FRAP was performed at spines of hippocampal neurons.
Neuroscience
High-Resolution Quantitative Immunogold Analysis of Membrane Receptors at Retinal Ribbon Synapses
Jun Zhang 1, Ronald S. Petralia 2, Ya-Xian Wang 2, Jeffrey S. Diamond 1
1Synaptic Physiology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 2Advanced Imaging Core, National Institute on Deafness and Other Communication Disorders, National Institutes of Health
The postembedding immunogold method is one of the most effective ways to provide high-resolution analyses of the subcellular localization of specific molecules. Here we describe a protocol to quantitatively analyze glutamate receptors at retinal ribbon synapses.
JoVE Hakkında
Telif Hakkı © 2020 MyJove Corporation. Tüm hakları saklıdır