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The Second Hospital of Harbin Medical University

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

An Optic Nerve Crush Injury Murine Model to Study Retinal Ganglion Cell Survival

Zhongshu Tang¹, Shuihua Zhang^1,2, Chunsik Lee¹, Anil Kumar¹, Pachiappan Arjunan¹, Yang Li¹, Fan Zhang¹, Xuri Li¹

¹National Eye Institute, NIH, ²Ophthalmology Department, The Second Hospital of Harbin Medical University

This protocol shows how to retrogradely label retinal ganglion cells, and how to subsequently make an optic nerve crush injury in order to analyze retinal ganglion cell survival and apoptosis. It is an experimental disease model for different types of optic neuropathy, including glaucoma.