University of North Carolina
2 ARTICLES PUBLISHED IN JoVE
Biology
Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c
Adam J. Kole 1, Elizabeth R.W. Knight 2, Mohanish Deshmukh 1
1Department of Cell and Developmental Biology, Neuroscience Center, University of North Carolina , 2Curriculum in Neurobiology, Neuroscience Center, University of North Carolina
In this protocol, we describe the direct cytoplasmic microinjection of cytochrome c protein into fibroblasts and primary sympathetic neurons. This technique allows for the introduction of cytochrome c protein into the cytoplasm of cells and mimics the release of cytochrome c from mitochondria, which occurs during apoptosis.
Bioengineering
Formulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique
Shrirang Karve 1,2, Michael E. Werner 1,2, Natalie D. Cummings 1,2, Rohit Sukumar 1,2, Edina C. Wang 1,2, Ying-Ao Zhang 1,2, Andrew Z. Wang 1,2
1Laboratory of Nano- and Translational Medicine, Department of Radiation Oncology, Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, 2Carolina Center for Nanotechnology Excellence, University of North Carolina
This article describes a nanoprecipitation method to synthesize polymer-based nanoparticles using diblock co-polymers. We will discuss the synthesis of diblock co-polymers, the nanoprecipitation technique, and potential applications.
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