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Trinity College Dublin

3 ARTICLES PUBLISHED IN JoVE

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Biology

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
Martin Caffrey 1, Christopher Porter 1
1Membrane Structural and Functional Biology Group, Schools of Biochemistry and Immunology and Medicine, Trinity College Dublin

Herein is described the procedure implemented in the Caffrey Membrane Structural and Functional Biology Group to set up manually crystallization trials of membrane proteins in lipidic mesophases.

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Biology

Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases
Dianfan Li 1, Coilín Boland 1, Kilian Walsh 1, Martin Caffrey 1
1Membrane Structural and Functional Biology Group, Schools of Medicine and Biochemistry & Immunology, Trinity College Dublin

Herein is described a robotic approach to high-throughput crystallization of membrane proteins in lipidic mesophases for use in structure determination using macromolecular X-ray crystallography. Three robots capable of handling the viscous and sticky protein-laden mesophase integral to the method are introduced.

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Biology

Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography
Dianfan Li 1, Coilín Boland 1, David Aragao 1, Kilian Walsh 1, Martin Caffrey 1
1Membrane Structural and Functional Biology Group, Schools of Medicine and Biochemistry & Immunology, Trinity College Dublin

Herein is described procedures implemented in the Caffrey Membrane Structural and Functional Biology Group to harvest and cryo-cool membrane protein crystals grown in lipidic cubic and sponge phases for use in structure determination using macromolecular X-ray crystallography.

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