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University of Sheffield

4 ARTICLES PUBLISHED IN JoVE

Bioengineering

Postproduction Processing of Electrospun Fibres for Tissue Engineering

Frazer J. Bye¹, Linge Wang², Anthony J. Bullock¹, Keith A. Blackwood¹, Anthony J. Ryan³, Sheila MacNeil¹

¹Materials Science and Engineering, University of Sheffield , ²Department of Biomedical Science, University of Sheffield , ³Department of Chemistry, University of Sheffield

Electrospun scaffolds can be processed post production for tissue engineering applications. Here we describe methods for spinning complex scaffolds (by consecutive spinning), for making thicker scaffolds (by multi-layering using heat or vapour annealing), for achieving sterility (aseptic production or sterilisation post production) and for achieving appropriate biomechanical properties.

Bioengineering

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Ílida Ortega¹, Farshid Sefat¹, Pallavi Deshpande¹, Thomas Paterson¹, Charanya Ramachandran³, Anthony J. Ryan², Sheila MacNeil¹, Frederik Claeyssens¹

¹Department of Materials Science and Engineering, University of Sheffield, ²Department of Chemistry, University of Sheffield, ³L. V. Prasad Eye Institute

We report a technique for the fabrication of micropockets within electrospun membranes in which to study cell behavior. Specifically, we describe a combination of microstereolithography and electrospinning for the production of PLGA (Poly(lactide-co-glycolide)) corneal biomaterial devices equipped with microfeatures.

Bioengineering

Production, Characterization and Potential Uses of a 3D Tissue-engineered Human Esophageal Mucosal Model

Nicola H. Green¹, Bernard M. Corfe², Jonathan P. Bury³, Sheila MacNeil¹

¹Department of Materials Science and Engineering, University of Sheffield, ²Department of Oncology and Insigneo Institute for in silico Medicine, University of Sheffield, ³Department of Histopathology, Sheffield Teaching Hospitals NHS Foundation Trust

This manuscript describes the production, characterization and potential uses of a tissue engineered 3D esophageal construct prepared from normal primary human esophageal fibroblast and squamous epithelial cells seeded within a de-cellularized porcine scaffold. The results demonstrate the formation of a mature stratified epithelium similar to the normal human esophagus.

Immunology and Infection

Establishing a Porcine Ex Vivo Cornea Model for Studying Drug Treatments against Bacterial Keratitis

Katarzyna Okurowska^1,2, Sanhita Roy³, Praveen Thokala⁴, Lynda Partridge^1,5, Prashant Garg³, Sheila MacNeil^1,6, Peter N. Monk^1,7, Esther Karunakaran^1,2

¹Sheffield Collaboratorium for Antimicrobial Resistance and Biofilms (SCARAB), University of Sheffield, ²Department of Chemical and Biological Engineering, University of Sheffield, ³Hyderabad Eye Research Foundation, L V Prasad Eye Institute, ⁴Health Economics and Decision Science, School of Health and Related Research, University of Sheffield, ⁵Department of Molecular Biology and Biotechnology, University of Sheffield, ⁶Department of Materials Science and Engineering, University of Sheffield, ⁷Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield

This article describes a step-by-step protocol to set up an ex vivo porcine model of bacterial keratitis. Pseudomonas aeruginosa is used as a prototypic organism. This innovative model mimics in vivo infection as bacterial proliferation is dependent on the ability of the bacterium to damage corneal tissue.