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Karlsruhe Institute of Technology - Campus North

1 ARTICLES PUBLISHED IN JoVE

JoVE Journal

A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System

Benjamin D. Weger¹, Meltem Weger¹, Nicole Jung², Christin Lederer¹, Stefan Bräse^1,3, Thomas Dickmeis¹

¹Institute of Toxicology and Genetics, Karlsruhe Institute of Technology - Campus North, ²Institute of Organic Chemistry, Karlsruhe Institute of Technology - Campus North, ³Institute of Organic Chemistry, Karlsruhe Institute of Technology - Campus South

We describe the procedure and data analysis of a chemical screening system for glucocorticoid stress hormone signaling using zebrafish larvae: the Glucocorticoid Responsive In vivo Zebrafish Luciferase activitY (GRIZLY) assay. The assay sensitively and specifically detects effects on glucocorticoid signaling by compounds that require metabolization or affect endogenous glucocorticoid production.