An Orthotopic Glioblastoma Mouse Model Maintaining Brain Parenchymal Physical Constraints and Suitable for Intravital Two-photon MicroscopyClément Ricard 1,2, Fabio Stanchi 1,3, Geneviève Rougon 1,2, Franck Debarbieux 1,2
1Developmental Biology Institute of Marseille, Aix Marseille University, 2European Research Center for Medical Imaging, Campus de la Timone, 3Vesalius Research Center, KU Leuven Campus Gasthuisberg
We have established a cortical orthotopic glioblastoma model in mice for intravital two-photon microscopy that recapitulates the biophysical constraints normally at play during the growth of the tumor. A chronic glass window replacing the skull above the tumor enables the follow-up of the tumor progression over time by two-photon microscopy.
