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The University of Texas Graduate School of Biomedical Sciences

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer's Patches Using Adoptive Transfer of Hematopoietic Progenitors

Haiyan S. Li¹, Stephanie S. Watowich^1,2

¹Department of Immunology, The University of Texas MD Anderson Cancer Center, ²The University of Texas Graduate School of Biomedical Sciences

This protocol describes experimental procedures to assess the differentiation of plasmacytoid dendritic cells in Peyer’s patch from common dendritic cell progenitors, using techniques involving FACS-mediated cell isolation, hydrodynamic gene transfer, and flow analysis of immune subsets in Peyer’s patch.