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Max-Planck-Institute for Molecular Biomedicine and Institute of Cell Biology

1 ARTICLES PUBLISHED IN JoVE

Biology

Live Cell Imaging of Primary Rat Neonatal Cardiomyocytes Following Adenoviral and Lentiviral Transduction Using Confocal Spinning Disk Microscopy

Takashi Sakurai^1,2, Anthony Lanahan², Melissa J. Woolls², Na Li², Daniela Tirziu², Masahiro Murakami²

¹Max-Planck-Institute for Molecular Biomedicine and Institute of Cell Biology, ²Department of Internal Medicine, Yale Cardiovascular Research Center and Section of Cardiovascular Medicine

This protocol describes a method of live cell imaging using primary rat neonatal cardiomyocytes following lentiviral and adenoviral transduction using confocal spinning disk microscopy. This enables detailed observations of cellular processes in living cardiomyocytes.