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Institut de Génomique Fonctionnelle, Montpellier

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Primary Culture of Mouse Dopaminergic Neurons

Florence Gaven^1,2,3, Philippe Marin^1,2,3, Sylvie Claeysen^1,2,3

¹CNRS, UMR-5203, Institut de Génomique Fonctionnelle, Montpellier, ²Inserm, U661, Montpellier, ³Universités de Montpellier

Dopaminergic neurons play a vital regulatory role in the brain. Their loss is associated with Parkinson's disease. In this video, we show how to generate primary cultures of central dopaminergic neurons from embryonic mouse mesencephalon. Such cultures are useful to study the extreme vulnerability of these neurons to various stresses.

Medicine

Longitudinal In Vivo Imaging of the Cerebrovasculature: Relevance to CNS Diseases

Margarita Arango-Lievano^1,2,3, Patrizia Giannoni^1,2,3, Sylvie Claeysen^1,2,3, Nicola Marchi^1,2,3, Freddy Jeanneteau^1,2,3

¹Inserm, U1191, Institute of Functional Genomics, ²CNRS, UMR-5203, ³Université de Montpellier

This manuscript describes a procedure to track the remodeling of the cerebrovasculature during amyloid plaque accumulation in vivo using longitudinal two-photon microscopy. A thinned-skull preparation enables the visualization of fluorescent dyes to assess the progression of cerebrovascular damage in a mouse model of Alzheimer's disease.