Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated MitochondriaSarah Pickles 1, Nathalie Arbour 2, Christine Vande Velde 2
1Department of Biochemistry, Université de Montréal, CRCHUM, 2Department of Neurosciences, Université de Montréal, CRCHUM
Described here is a method to detect and quantify mitochondrial outer membrane proteins by immunolabeling of mitochondria isolated from rodent tissue and analysis by flow cytometry. This method can be extended to assess functional aspects of mitochondrial subpopulations.
