Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via ElectroporationLouise Aigrain 1,2, Marko Sustarsic 1, Robert Crawford 1, Anne Plochowietz 1, Achillefs N. Kapanidis 1
1Clarendon Laboratory, Department of Physics, University of Oxford, 2Wellcome Trust Sanger Institute, Genome Center
Studies of biomolecules in vivo are crucial for understanding molecular function in a biological context. Here we describe a novel method allowing the internalization of fluorescent biomolecules, such as DNA or proteins, into living microorganisms. Analysis of in vivo data recorded by fluorescence microscopy is also presented and discussed.
