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Medical University of South Carolina, College of Medicine

1 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Culture of Embryonic Mouse Cochlear Explants and Gene Transfer by Electroporation

Khujista D. Haque¹, Atul K. Pandey¹, Matthew W. Kelley², Chandrakala Puligilla¹

¹Department of Pathology and Laboratory Medicine, Medical University of South Carolina, College of Medicine, ²Laboratory of Cochlear Development, NIDCD, NIH

We present a method that describes isolation and culture of cochlear explants from embryonic mouse inner ear. We also demonstrate a method for gene transfer into cochlear explants via square-wave electroporation. The in vitro explant culture coupled with gene transfer technique enables researchers to study the effects of altering gene expression during development.