Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal PlasmidsViviana Meraviglia *1, Alessandra Zanon *1, Alexandros A. Lavdas 1, Christine Schwienbacher 1, Rosamaria Silipigni 2, Marina Di Segni 2, Huei-Sheng Vincent Chen 3, Peter P. Pramstaller 1, Andrew A. Hicks 1, Alessandra Rossini 1
1Center for Biomedicine, European Academy Bozen/Bolzano (EURAC), 2Laboratory of Medical Genetics, Fondazione IRCCS Ca´ Granda, Ospedale Maggiore Policlinico, 3Del E. Webb Center for Neuroscience, Aging & Stem Cell Research, Sanford-Burnham Medical Research Institute
Induced pluripotent stem cells (iPSCs) represent a source of patient-specific tissues for clinical applications and basic research. Here, we present a detailed protocol to reprogram human peripheral blood mononuclear cells (PBMNCs) obtained from frozen buffy coats into viral-free iPSCs using non-integrating episomal plasmids.
