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German Heart Center Munich, Technische Universität München

2 ARTICLES PUBLISHED IN JoVE

Medicine

Heterotopic Cervical Heart Transplantation in Mice

Thomas Ratschiller^1,4, Marcus-André Deutsch¹, Julia Calzada-Wack², Frauke Neff², Christiane Roesch³, Ralf Guenzinger³, Ruediger Lange³, Markus Krane³

¹Department of Cardiovascular Surgery, Division of Experimental Surgery, German Heart Center Munich, Technische Universität München, ²Institute of Pathology, Helmholtz Zentrum Munich, ³Department of Cardiovascular Surgery, Division of Experimental Surgery, German Heart Center Munich, Technische Universität München, ⁴Department of Cardiovascular and Thoracic Surgery, General Hospital Linz

The cuff technique shortens and facilitates the model of heterotopic cervical heart transplantation in mice by avoiding technically challenging suture anastomoses of small vessels. The technical details shown in this video paper should allow researches to establish this model in their laboratories.

Biology

Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles

Harald Lahm^*1, Stefanie A. Doppler^*1, Martina Dreßen¹, Klaudia Adamczyk¹, Marcus-André Deutsch¹, Hanna Ulrich², Matthias Schiemann^2,3, Rüdiger Lange^1,4, Markus Krane^1,4

¹Department of Cardiovascular Surgery, German Heart Center Munich, Technische Universität München, ²Institute for Medical Microbiology, Immunology, and Hygiene, Technische Universität München, ³Clinical Cooperation Groups: "Antigen-specific Immunotherapy" and "Immune Monitoring", Helmholtz Center Munich (Neuhererg), Technische Universität München, ⁴DZHK (German Center for Cardiovascular Research) – Partner site Munich Heart Alliance

This manuscript describes a novel technique which allows the detection of intracellular gene expression after endocytosis of fluorescence-labeled nanoparticles directly in live cells. The method does not require manipulation of the cells and is not restricted with respect to the target gene or species.