The Hospital for Sick Children, Toronto
2 ARTICLES PUBLISHED IN JoVE
Medicine
Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation
J. Moritz Kaths 1,2, Vinzent N. Spetzler 1, Nicolas Goldaracena 1, Juan Echeverri 1, Kristine S. Louis 1, Daniel B. Foltys 3, Mari Strempel 4, Paul Yip 5, Rohan John 5, Istvan Mucsi 1, Anand Ghanekar 1, Darius Bagli 6,7, Lisa Robinson 2, Markus Selzner 1
1Multi Organ Transplant Program, Department of Surgery, Toronto General Hospital, 2Division of Nephrology, The Hospital for Sick Children, Toronto, 3Department of General, Visceral & Transplant Surgery, University Medical Center Mainz, 4Department of Abdominal, Vascular & Transplant Surgery, Merheim Medical Center Cologne, 5Laboratory Medicine & Pathobiology, Toronto General Hospital, 6Departments of Surgery (Urology) & Physiology, The Hospital for Sick Children, Toronto, 7Developmental & Stem Cell Biology, The Hospital for Sick Children, Toronto
The severe organ shortage has resulted in increased use of marginal kidney grafts for transplantation. This has triggered interest in alternative storage methods, since marginal grafts especially tolerate cold storage poorly. The technique of normothermic ex vivo kidney perfusion (NEVKP) represents a novel preservation method for kidney grafts prior to transplantation.
Medicine
Heterotopic Renal Autotransplantation in a Porcine Model: A Step-by-Step Protocol
J. Moritz Kaths 1,2, Juan Echeverri 1,3, Nicolas Goldaracena 1, Kristine S. Louis 1, Paul Yip 4, Rohan John 4, Istvan Mucsi 5, Anand Ghanekar 1, Darius Bagli 6, Markus Selzner 1, Lisa A. Robinson 2
1Multi Organ Transplant Program, Department of Surgery, Toronto General Hospital, 2Division of Nephrology, The Hospital for Sick Children, 3Programa de Doctorat en Medicina, La Universitat Autónoma de Barcelona, 4Laboratory Medicine and Pathobiology, Toronto General Hospital, 5Department of Medicine, Toronto General Hospital, 6Departments of Surgery (Urology) & Physiology, Developmental & Stem Cell Biology, The Hospital for Sick Children
Porcine models of organ transplantation provide an important platform to study mechanisms of organ preservation. This article describes a heterotopic porcine renal autotransplantation model, which allows investigating new approaches to improve the outcome of transplantation using marginal kidney grafts.
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