St John's University

1 ARTICLES PUBLISHED IN JoVE

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Biology

In Vitro Assay to Measure Phosphatidylethanolamine Methyltransferase Activity
Rachel Zufferey 1
1Department of Biological Sciences, St John's University

The present report describes an in vitro enzymatic assay to measure phosphatidylethanolamine methyltransferase activity using Leishmania cell extracts. This assay is based on the transfer of a radioactive methyl group from S-[Methyl-3H]adenosyl-L-methionine onto endogenous phosphatidylethanolamine.

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