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Interuniversity Cardiology Institutes of the Netherlands (ICIN)

3 ARTICLES PUBLISHED IN JoVE

Medicine

Myocardial Infarction and Functional Outcome Assessment in Pigs

Stefan Koudstaal^1,2, Sanne J. Jansen of Lorkeers¹, Johannes M.I.H. Gho¹, Gerardus P.J van Hout¹, Marlijn S. Jansen¹, Paul F. Gründeman¹, Gerard Pasterkamp^1,2, Pieter A. Doevendans^1,2, Imo E. Hoefer^*1, Steven A.J. Chamuleau^*1

¹Department of Cardiology, Division Heart and Lungs, University Medical Center Utrecht, ²Interuniversity Cardiology Institute of the Netherlands

This protocol describes the porcine myocardial infarction (MI) model using a 90 min closed-chest coronary balloon occlusion of the left anterior descending artery (LAD), followed by reperfusion. Furthermore, the protocol for several outcome parameters, such as cardiac function, hemodynamics, microvascular resistance, and infarct size, are also presented.

Medicine

Primary Outcome Assessment in a Pig Model of Acute Myocardial Infarction

Guilielmus H.J.M. Ellenbroek¹, Gerardus P.J. van Hout^1,2, Leo Timmers^1,2, Pieter A. Doevendans^2,4, Gerard Pasterkamp^1,3, Imo E. Hoefer^1,3

¹Department of Experimental Cardiology, University Medical Center Utrecht, ²Department of Cardiology, University Medical Center Utrecht, ³Department of Clinical Chemistry and Hematology, University Medical Center Utrecht, ⁴Interuniversity Cardiology Institutes of the Netherlands (ICIN)

Reliable and accurate outcome assessment is the key for translation of preclinical therapies into clinical treatment. The current paper describes how to assess three clinically relevant primary outcome parameters of cardiac performance and damage in a pig acute myocardial infarction model.

Medicine

3D Whole-heart Myocardial Tissue Analysis

Hans Thijs Van den Broek¹, Leon De Jong^1,2, Pieter A. Doevendans¹, Steven A.J. Chamuleau¹, Frebus J. Van Slochteren^*1, René Van Es^*1

¹Department of Cardiology, Division Heart and Lungs, University Medical Center Utrecht, ²MIRA Institute, University Twente

This protocol describes a novel method for the 3D comparison of whole-heart myocardial tissue with MRI. This is designed for the accurate assessment of intramyocardial injections in the infarct border zone of a chronic porcine model of myocardial infarction.