Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density LipoproteinMulugeta Seneshaw 1, Faridoddin Mirshahi 1, Hae-Ki Min 1, Amon Asgharpour 1, Shervin Mirshahi 1, Kalyani Daita 1, Sherry Boyett 1, Prasanna K. Santhekadur 2, Michael Fuchs 1, Arun J. Sanyal 1
1Division of Gastroenterology, Hepatology and Nutrition, Virginia Commonwealth University, 2McGuire Veterans Affairs Medical Center, Research BC-101
MicroRNAs play an important regulatory role and are emerging as novel therapeutic targets for various human diseases. It has been shown that miRNAs are carried in high density lipoproteins. We have developed a simplified method to rapidly isolate purified HDL suitable for miRNA analysis from human plasma.
