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Institute of Biotechnology, Berlin University of Technology

2 ARTICLES PUBLISHED IN JoVE

JoVE Journal

The Multi-organ Chip - A Microfluidic Platform for Long-term Multi-tissue Coculture

Eva-Maria Materne^*1, Ilka Maschmeyer^*1,2, Alexandra K. Lorenz¹, Reyk Horland^1,2, Katharina M. S. Schimek¹, Mathias Busek³, Frank Sonntag³, Roland Lauster¹, Uwe Marx^1,2

¹Medical Biotechnology, Technische Universität Berlin, ²TissUse GmbH, ³Fraunhofer IWS

Here, we present a protocol to coculture primary cells, tissue models and punch biopsies in a microfluidic multi-organ chip for up to 28 days. Human dermal microvascular endothelial cells, liver aggregates and skin biopsies were successfully combined in a common media circulation.

Cancer Research

Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2

Thomas Hiller¹, Viola Röhrs¹, Eva-Maria Dehne², Anke Wagner^1,3, Henry Fechner¹, Roland Lauster², Jens Kurreck¹

¹Department of Applied Biochemistry, Institute of Biotechnology, Berlin University of Technology, ²Department of Medical Biotechnology, Institute of Biotechnology, Berlin University of Technology, ³Department of Bioprocess Engineering, Institute of Biotechnology, Berlin University of Technology

The recellularized extracellular matrix of a decellularized rat liver can be used as a humanized, three-dimensional ex vivo model to study the distribution and transgene expression of a virus or viral vector.