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Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Bottlenose Dolphin (Tursiops truncatus) Spermatozoa: Collection, Cryopreservation, and Heterologous In Vitro Fertilization

María Jesús Sánchez-Calabuig^1,2, Francisco Alberto García-Vázquez³, Ricardo Laguna-Barraza¹, Carlos Barros-García⁴, Daniel García-Parraga⁵, Dimitrios Rizos¹, Alfonso Gutiérrez Adan¹, José Félix Pérez-Gutíerrez²

¹Department of Animal Reproduction, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), ²Department of Animal Medicine and Surgery, School of Veterinary Medicine, Universidad Complutense de Madrid, ³Department of Physiology, Faculty of Veterinary Science, University of Murcia, Campus Mare Nostrum, ⁴Mundomar, Benidorm, ⁵Veterinary Services, L'Oceanográfic, Ciudad de las Artes y las Ciencias, Junta de Murs i Vals, s/n, 46013

Here, we present protocols that have been successfully used for dolphin spermatozoa collection, cryopreservation, and heterologous IVF performance using bovine oocytes.

Environment

Cereal Crop Ear Counting in Field Conditions Using Zenithal RGB Images

José A. Fernandez-Gallego^1,2,3, María Luisa Buchaillot^1,2, Adrian Gracia-Romero^1,2, Thomas Vatter^1,2, Omar Vergara Diaz^1,2, Nieves Aparicio Gutiérrez⁴, María Teresa Nieto-Taladriz⁵, Samir Kerfal⁶, Maria Dolors Serret^1,2, José Luis Araus^1,2, Shawn C. Kefauver^1,2

¹Plant Physiology Section, Faculty of Biology, University of Barcelona, ²Agrotecnio, ³Programa de Ingeniería Electrónica, Facultad de Ingeniería, Universidad de Ibagué, ⁴Instituto Tecnológico Agrario de Castilla y León (ITACyL), ⁵Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), ⁶Syngenta Spain

We present a protocol for counting durum wheat and barley ears, using natural color (RGB) digital photographs taken in natural sunlight under field conditions. With minimal adjustments for camera parameters and some environmental condition limitations, the technique provides precise and consistent results across a range of growth stages.