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University of Chinese Academy of Sciences, Chinese Academy of Science

4 ARTICLES PUBLISHED IN JoVE

Behavior

An Automated Rapid Iterative Negative Geotaxis Assay for Analyzing Adult Climbing Behavior in a Drosophila Model of Neurodegeneration

Wenze Cao^*1,2,3, Li Song^*1,2,3, Jingjing Cheng^1,2,3, Na Yi^1,2,3, Luyi Cai^1,2,3, Fu-de Huang^4,5, Margaret Ho^1,2,3

¹Research Center for Translational Medicine, Tongji University School of Medicine, ²Key Laboratory of Arrhythmias of the Ministry of Education of China, Shanghai East Hospital, Tongji University School of Medicine, ³Department of Anatomy and Neurobiology, Tongji University School of Medicine, ⁴Shanghai Advanced Research Institute, University of Chinese Academy of Sciences, Chinese Academy of Sciences, ⁵Sino-Danish College, University of Chinese Academy of Sciences, Chinese Academy of Sciences

This step-by-step protocol analyzes Drosophila negative geotaxis behavior using an automated multi-cylinder system that hosts hundreds of flies and synchronizes their action by an electric motor. Upon synchronization, fly negative geotaxis behavior is assayed, digitally recorded, and analyzed using the self-designed RflyDetection software.

Neuroscience

An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice

Nastasia K-H Lim^1,2, Visse Moestrup^3,4, Xiao Zhang¹, Wen-An Wang⁵, Arne Møller^3,4,6, Fu-De Huang^1,4

¹Shanghai Advanced Research Institute, University of Chinese Academy of Sciences, Chinese Academy of Science, ²Shanghai Institute of Materia Medica, University of Chinese Academy of Sciences, Chinese Academy of Science, ³Center of Functionally Integrative Neuroscience, Department of Clinical Medicine, Aarhus University, ⁴Sino-Danish Center for Education and Research (SDC), ⁵Department of Neurology, Xinhua Hospital Chongming Branch Affiliated to Shanghai Jiao Tong University School of Medicine, ⁶Department of Nuclear Medicine and PET-centre, Aarhus University Hospital

This protocol describes an improved technique for the abundant collection of cerebrospinal fluid (CSF) with no contamination from blood. With greater sample collection and purity, more analyses can be performed using CSF to further our understanding of diseases that affect the brain and spinal cord.

Medicine

Directed Induction of Retinal Organoids from Human Pluripotent Stem Cells

Xiao Zhang¹, Zi-Bing Jin¹

¹Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology & Visual Science Key Laboratory

Using a self-organizing method, we develop a protocol with the addition of COCO that could significantly increase the generation of photoreceptors.

Cancer Research

Reconstruct Human Retinoblastoma In Vitro

Xiao Zhang¹, Zi-Bing Jin¹

¹Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology & Visual Science Key Laboratory

We describe a method for generating human retinoblastoma (RB) by introducing biallelic RB1 mutations in human embryonic stem cells (hESC). RB cell lines could also be successfully cultured using the isolated RB in a dish.