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Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology

5 ARTICLES PUBLISHED IN JoVE

Developmental Biology

A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro

Zhaofeng Jia^1,2, Yujie Liang³, Bin Ma^4,5, Xiao Xu^2,6, Jianyi Xiong², Li Duan², Daping Wang^1,2

¹Guangzhou Medical University, ²Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopeadic Engineering, Department of Orthopedics, Shenzhen Second People's Hospital (The First Hospital Affiliated to Shenzhen University), ³Department of Chemistry, The Chinese University of Hong Kong, ⁴School of Biomedical Engineering, Shanghai Jiao Tong University, ⁵Renji Hospital Clinical Stem Cell Research Center, Shanghai Jiao Tong University School of Medicine, ⁶Shantou University Medical College

We present a method to quantify DNA methylation based on the 5-methylcytosine (5-mC) dot blot. We determined the 5-mC levels during chondrocyte dedifferentiation. This simple technique could be used to quickly determine the chondrocyte phenotype in ACI treatment.

Neuroscience

Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization

Xiao Xu¹, Yinwei You^1,2, Long Zhang¹

¹Department of Entomology, China Agricultural University, ²Bio-tech Research Center, Shandong Academy of Agricultural Sciences

This paper describes a detailed and highly effective RNA in situ hybridization protocol particularly for low-level expressed Odorant Receptor (OR) genes, as well as other genes, in insect antennae using digoxigenin (DIG)-labeled or biotin-labeled probes.

Biology

Magnetic-Activated Cell Sorting Strategies to Isolate and Purify Synovial Fluid-Derived Mesenchymal Stem Cells from a Rabbit Model

Zhaofeng Jia^1,2,3, Yujie Liang^4,5, Xingfu Li^2,3, Xiao Xu^1,2,3, Jianyi Xiong^2,3, Daping Wang^1,2,3, Li Duan^2,3

¹Postgraduate institution, Guangzhou Medical University, ²Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, ³Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopaedic Engineering, Shenzhen Second People's Hospital (The First Hospital Affiliated to Shenzhen University), ⁴Department of Chemistry, Chinese University of Hong Kong, ⁵Shenzhen Kangning Hospital, Shenzhen Mental Health Center

This article presents a simple and economic protocol for the straightforward isolation and purification of mesenchymal stem cells from New Zealand white rabbit synovial fluid.

JoVE Journal

Thermocapillary Convection Space Experiment on the SJ-10 Recoverable Satellite

Li Duan^*1,2, Yongli Yin^*3, Jia Wang^*1, Qi Kang^1,2, Di Wu¹, Huan Jiang¹, Pu Zhang¹, Liang Hu¹

¹National Microgravity Laboratory, Institute of Mechanics, Chinese Academy of Sciences, ²School of Engineering Sciences, University of Chinese Academy of Sciences, ³China Astronaut Research and Training Center

A protocol for the space payload design, the space experiment on thermocapillary convection, and analyses of experimental data and images are presented in this paper.

Biology

Large-Scale Preparation of Synovial Fluid Mesenchymal Stem Cell-Derived Exosomes by 3D Bioreactor Culture

Li Duan^1,2, Xingfu Li^1,2, Xiao Xu^1,2, Limei Xu^1,2, Daping Wang^1,2, Kan Ouyang^1,2, Yujie Liang^1,3

¹Department of Orthopedics, The First affiliated hospital of Shenzhen University, Shenzhen Second People's Hospital, ²Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen Second People's Hospital, ³Department of Child and Adolescent Psychiatry, Shenzhen Kangning Hospital, Shenzhen Mental Health Center, Shenzhen Key Laboratory for Psychological Healthcare & Shenzhen Institute of Mental Health

Here, we present a protocol to produce a large number of GMP-grade exosomes from synovial fluid mesenchymal stem cells using a 3D bioreactor.