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Institute of Analytical Chemistry of the Czech Academy of Sciences

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion

Miroslava Stastna^1,2,3, Amandine Thomas¹, Juliana Germano¹, Somayeh Pourpirali¹, Jennifer E. Van Eyk^1,2, Roberta A. Gottlieb¹

¹The Smidt Heart Institute, Cedars-Sinai Medical Center, ²Advanced Clinical Biosystems Research Institute, Cedars-Sinai Medical Center, ³Institute of Analytical Chemistry of the Czech Academy of Sciences

Here we present a protocol to perform polysome profiling on the isolated perfused mouse heart. We describe methods for heart perfusion, polysome profiling, and analysis of the polysome fractions with respect to mRNAs, miRNAs, and the polysome proteome.